STUDIES ON IMMUNE HUMAN HEMOLYSIS
نویسندگان
چکیده
منابع مشابه
Studies on Immune Human Hemolysis
The Donath-Landsteiner reaction was studied using low and high titer antisera and purified antibody, normal and PNH erythrocytes, and human serum complement. The requirement for complement in both the cold and warm phases of the reaction depended upon the level of antibody used and the sensitivity of the cells to hemolytic antibodies. Complement was not necessary in the cold phase using PNH cel...
متن کاملKinetic Studies on Immune Hemolysis
A quantitative method for the study of the kinetics of immune hemolysis is described. In the presence of excess C' and limited amounts of antibody the kinetics of hemolysis resemble those of enzymatic processes. Assays of hemolytic antibody should therefore be based on velocity measurements rather than end point titrations.
متن کاملStudies on Immune Human Hemolysis I. the Kinetics of the Donath-landsteiner Reaction
Serum complement is of potential importance to clinical medicine and to human biology in immune reactions and hypersensitivity states. However, most studies on the characteristics and mechanism of action of complement have been performed on systems largely of non-human origin (1, 2). The present studies were undertaken to study a specific immune human hemolytic disorder and to use this as a mod...
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Additional experimental work on the subject of eosin hemolysis has been carried out. This indicates that red cells may be protected against the toxic action of eosin in sunlight by the presence of inorganic reducing agents. It is pointed out that a marked parallelism exists between the substances which react with the Folin and Denis reagent and the compounds which afford protection to red cells...
متن کاملThe Reaction Mechanism of Human C5 in Immune Hemolysis
The data presented here indicate that the C5 reaction step may proceed via the specific attachment of C5 to EAC1,4,2,3 and the formation of a hemolytically active C5 intermediate complex. During this process only a minor proportion (less than 4%) of C5 offered to EAC1,4,2,3 becomes bound, although the remaining C5 also participates in the reaction as evidenced by its inactivation in the fluid p...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1961
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.113.1.193